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Journal: STAR Protocols
Article Title: Protocol for chromatin immunoprecipitation using isolated antheridia of Marchantia polymorpha
doi: 10.1016/j.xpro.2026.104388
Figure Lengend Snippet: An example of ChIP-qPCR results (A) Tissue expression pattern of alpha-tubulin genes in M. polymorpha . Transcripts per million (TPM) values are log2-transformed after adding a pseudo count of 1 to avoid negative values for expression levels. Raw TPM values are obtained from MarpolBase Expression (MBEX, https://marchantia.info/mbex/ ). (B) Schematic diagrams of genomic regions of Mp TUA5 , Mp TUB4 , and Mp TUA3 . Black closed boxes represent exons. PCR amplicons used for ChIP-qPCR are indicated as short bars with letters, a and b. The DUO1 consensus DNA-binding motif (5′-RRCSGTT-3′) is shown as a blue vertical line. (C) ChIP-qPCR analysis showing the enrichment of MpDUO1 on the TSS of Mp TUA5 and Mp TUB4 . pro Mp DUO1 : mCitrine-NLS plant was used as a negative control. The ChIP DNA was quantified by qPCR, and the DNA enrichment was shown as a percentage of input DNA (%input). Mp TUA3 , whose expression level is very low in antheridia and sperm was used as a negative control. Error bars indicate SD of three biological replicates (n = 3).
Article Snippet: Note: This protocol used the
Techniques: ChIP-qPCR, Expressing, Transformation Assay, Binding Assay, Negative Control